Two logistic regression classifiers were implemented for the time series classification task using as features the area under the curve and skewness in the first classifier and skewness and kurtosis in the second classifier. Once we have found both decision boundaries in two different feature spaces by training using 120 time series, the decision boundaries were tested over 12 image stacks through a python graphical user interface (GUI), generating binary images where white pixels correspond to cells and the black ones to background. Results show that area-skewness classifier reduces the time an expert dedicates in locating cells by up to 75% in some stacks versus a 92% for the kurtosis-skewness classifier, this evaluated on the number of regions the method found. Due to the promising results, we expect that this method will be improved adding more relevant features to the classifier.
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Erika González, Lucía Medina, Mathieu Hautefeuille, Tatiana Fiordelisio, "Segmentation and analysis of mouse pituitary cells with graphic user interface (GUI)," Proc. SPIE 10497, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVI, 104971T (20 February 2018);