30 March 2018 Front Matter: Volume 10498
This PDF file contains the front matter associated with SPIE Proceedings Volume 10498, including the Title Page, Copyright information, Table of Contents, Introduction, and Conference Committee listing.

The papers in this volume were part of the technical conference cited on the cover and title page. Papers were selected and subject to review by the editors and conference program committee. Some conference presentations may not be available for publication. Additional papers and presentation recordings may be available online in the SPIE Digital Library at SPIEDigitalLibrary.org.

The papers reflect the work and thoughts of the authors and are published herein as submitted. The publisher is not responsible for the validity of the information or for any outcomes resulting from reliance thereon.

Please use the following format to cite material from these proceedings:

Author(s), “Title of Paper,” in Multiphoton Microscopy in the Biomedical Sciences XVIII, edited by Ammasi Periasamy, Peter T. C. So, Karsten König, Xiaoliang S. Xie, Proceedings of SPIE Vol. 10498 (SPIE, Bellingham, WA, 2018) Seven-digit Article CID Number.

ISSN: 1605-7422

ISSN: 2410-9045 (electronic)

ISBN: 9781510614819

ISBN: 9781510614826 (electronic)

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Numbers in the index correspond to the last two digits of the seven-digit citation identifier (CID) article numbering system used in Proceedings of SPIE. The first five digits reflect the volume number. Base 36 numbering is employed for the last two digits and indicates the order of articles within the volume. Numbers start with 00, 01, 02, 03, 04, 05, 06, 07, 08, 09, 0A, 0B…0Z, followed by 10-1Z, 20-2Z, etc.

Akiyama, Toshihiro, 1P

Alemán Hérnandez, Felipe Ademir, 24

An, Yitai, 1I

Andersen, Peter E., 20

Artal, Pablo, 10

Ávila, Francisco, 10

Baldacchini, Tommaso, 0K

Barbieri, Beniamino, 0Q, 0W

Batista, Ana, 14

Becker, Wolfgang, 06

Bélanger, Samuel, 0X

Benda, Aleš, 06

Bijlsma, Hielke, 1X

Bobrov, Nikolai V., 27

Bonora, S., 1X

Börsch, Michael, 0T

Breunig, Hans Georg, 14

Breymayer, J., 0A

Brinkmann, Maximilian, 22, 23

Bueno, Juan, 10

Cagalinec, M., 0G

Capecchi, Mario R., 16

Castello, Marco, 2U

Castonguay, A., 2O

Cha, Yu-Rok, 2J

Chandler, Andrea, 37

Chandler, Lynn, 37

Chen, Congping, 1Q

Chen, Shean-Jen, 2D

Cheng, Pan, 28

Chiang, Tsung-Yen, 2D

Chiu, Daniel T., 2Y

Chorvat, D., Jr., 0G

Choubal, Aakash M., 2Z

Coskun, Ulas, 0Q, 0W

Coto Hernández, Iván, 2U

Couderc, Vincent, 1P, 2J

Cunderlikova, B., 0G

Damseh, R., 2O

Darafsheh, Arash, 33

Das, Subir, 2C

Delafontaine-Martel, P., 2O

Deng, Fengyuan, 34

Diaspro, Alberto, 2U

Dimopoulos, Konstantinos, 20

Ding, Changqin, 34

Dudenkova, Varvara V., 04, 07, 27

Dunn, Andrew K., 1V, 2Y

Elagin, Vadim, 04

Enger, Jonas, 24

Ericson, Marica B., 24, 25

Fallnich, Carsten, 22, 23

Fang, Yi-Cheng, 2L

Farewell, Anne, 25

Fellbaum, Carl, 2W

Fisher, Jon, 2W

Gaillard, Paul, 2W

Gaunt, Nicholas P., 36

Gavrina, Alena I., 07

Girouard, Hélène, 0X

Grisshammer, Reinhard, 0T

Guru, Akash, 16

Hanstorp, Dag, 24

Hase, Eiji, 2M

Hassan, Ahmed M., 1V, 2Y

He, Hao, 28

He, Sicong, 1I, 1Q

Heitkamp, Thomas, 0T

Hellwig, Tim, 22, 23

Ho, Bo-Wei, 2C

Horilova, J., 0G

Hsu, Chia-Wei, 2D

Hung, Jui-Hung, 2L

Hwang, Wonsang, 2G

Ignatova, Nadezgda, 04

Inoko, Akihito, 1P

Ito, Yoko, 2K

James, Jeemol, 24, 25

Jarrett, Jeremy W., 1V, 2Y

Jowett, Nate, 2U

Kabir, Mohammad M., 2Z

Kaji, Yuichi, 1P, 2J

Kalinina, S., 0A

Kano, Hideaki, 1P, 2J

Kao, Fu-Jen, 2C

Kawakami, Ryosuke, 2L

Kim, Dongeun, 2G

Kim, Dug Young, 2G

Kobayashi, Tsubasa, 1P, 2J

König, Karsten, 14

Kozawa, Yuichi, 2L

Kurokawa, Kazuo, 2K

Kuznetsova, Daria S., 27

Lai, Feng-Jie, 2D

Lanzano, Luca, 2U

Lee, Hsien-Ming, 0Q

Lefebvre, J., 2O

Leproux, Philippe, 1P, 2J

Lesage, Frédéric, 0X, 1Y, 2O

Li, Baoqiang, 1Y

Li, Bo, 16, 2A

Li, Xuesong, 1I, 1Q

Li, Yan, 1Q

Liao, Shih-Chu Jeff, 0Q, 0W

Lilge, L., 0A

Liu, Yen-Liang, 2Y

Lu, Xuecong, 0X, 1Y

Lukina, Maria, 04

Lukyanov, Konstantin A., 07

Maeda, Yasuhiro, 2K

Mak, Ho Yi, 1Q

Mandel, A., 0A

Marcek Chorvatova, A., 0G

Marti, Dominik, 20

Mikhaylov, Alexander, 30

Miller, David R., 1V, 2Y

Moeini, Mohammad, 0X, 1Y

Moger, Julian, 36

Moon, Sucbei, 2G

Mortensen, Luke J., 33

Nakano, Akihiko, 2K

Nam, Hyung-Song, 16

Nemoto, Tomomi, 2L

Nurmalasari, Ni Putu Dewi, 2W

Ogura, Yuki, 2M

Orlinskay, Natalia, 04

Oshika, Tetsuro, 1P, 2J

Otomo, Kohei, 2L

Ouzounov, Dimitre G., 16

Padia, Faheem, 36

Periasamy, Ammasi, 37

Perillo, Evan P., 1V, 2Y

Phang, Sendy, 33

Pozzi, P., 1X

Qin, Zhongya, 1Q

Qiu, Hailin, 0Q

Qu, Jianan Y., 1I, 1Q

Quintavalla, M., 1X

Ramanujan, V. Krishnan, 0B

Rebane, Aleksander, 30

Rodimova, Svetlana A., 27

Rothe, Sebastian, 24

Rück, A., 0A

Sakadžić, Sava, 1Y

Sato, Shunichi, 2L

Sawada, Kazuaki, 2L

Schäfer, P., 0A

Scheslavsky, Vladislav, 04

Sergeeva, Tatiana F., 07

Shirmanova, Marina V., 04, 07

Shlivko, Irena, 04

Simpson, Garth J., 34

Sinefeld, David, 16, 2A

Sivaguru, Mayandi, 2Z

Smith, Steve, 2W

Studier, Hauke, 06

Subramaniam, Sen, 2W

Sun, Yuansheng, 0Q, 0W

Tabatabaei, Maryam, 0X

Tanaka, Yuji, 2M

Tang, Wanyi, 28

Tardif, P., 2O

Tehrani, Kayvan Forouhesh, 33

Thomsen, Hanna, 24, 25

Thomson, Niall, 36

Tortarolo, Giorgio, 2U

Toussaint, Kimani C., Jr., 2Z

Ulcickas, James R. W., 34

Verhaegen, Michelle, 1X

Verstraete, Hans, 1X

Vicidomini, Giuseppe, 2U

von Arnim, C., 0A

von Einem, B., 0A

Wada, Satoshi, 2K

Wang, Mengran, 16, 2A

Wang, Tianyu, 16

Warden, Melissa R., 16

Wetzker, Cornelia, 06

Won, Youngjae, 2G

Wu, Chunyan, 16, 2A

Wu, Xu, 2Y

Wu, Zhenguo, 1I

Xia, Fei, 2A

Xu, Chris, 16, 2A

Xu, Shihan, 2Y

Yamashita, Toyonobu, 2M

Yasui, Takeshi, 2M

Yeh, Hsin-Chih, 2Y

Yokoyama, Hiroyuki, 2L

Yu, Jiangbo, 2Y

Zadoyan, Ruben, 0K

Zagainov, Vladimir E., 27

Zagaynova, Elena V., 04, 07, 27

Zeytunyan, Aram, 0K

Zhang, Cong, 0X, 1Y

Conference Committee

Symposium Chairs

  • James G. Fujimoto, Massachusetts Institute of Technology (United States)

  • R. Rox Anderson, Wellman Center for Photomedicine, Massachusetts General Hospital (United States) and Harvard Medical School (United States)

Program Track Chairs

  • Ammasi Periasamy, University of Virginia (United States)

  • Daniel L. Farkas, University of Southern California (United States) and SMI (United States)

Conference Chairs

  • Ammasi Periasamy, University of Virginia (United States)

  • Peter T. C. So, Massachusetts Institute of Technology (United States)

  • Karsten König, Universität des Saarlandes (Germany)

  • Xiaoliang S. Xie, Harvard University (United States) and Peking University (China)

Conference Program Committee

  • Holly Aaron, University of California, Berkeley (United States)

  • Margarida Barroso, Albany Medical College (United States)

  • Wolfgang Becker, Becker & Hickl GmbH (Germany)

  • Alberto Diaspro, Istituto Italiano di Tecnologia (Italy)

  • Chen-Yuan Dong, National Taiwan University (Taiwan)

  • Paul J. Campagnola, University of Wisconsin-Madison (United States)

  • Ji-Xin Cheng, Purdue University (United States)

  • Kevin W. Eliceiri, University of Wisconsin-Madison (United States)

  • Scott Fraser, University of Southern California (United States)

  • Paul M. W. French, Imperial College London (United Kingdom)

  • Katsumasa Fujita, Osaka University (Japan)

  • Enrico Gratton, University of California, Irvine (United States)

  • Min Gu, RMIT University (Australia)

  • Stefan W. Hell, Max-Planck-Institut für Biophysikalische Chemie (Germany)

  • Fu-Jen Kao, National Yang-Ming University (Taiwan)

  • Arnd K. Krueger, Spectra-Physics (United States)

  • Joseph R. Lakowicz, University of Maryland School of Medicine (United States)

  • Steve M. McDonald, Coherent, Inc. (United States)

  • Wei Min, Columbia University (United States)

  • Junle Qu, Shenzhen University (China)

  • Angelika C. Rueck, Universität Ulm (Germany)

  • Yuansheng Sun, ISS, Inc. (United States)

  • Steven S. Vogel, National Institutes of Health (United States)

  • Chris Xu, Cornell University (United States)

  • Elena V. Zagaynova, Nizhny Novgorod State Medical Academy (Russian Federation)

  • Bernhard Zimmermann, Carl Zeiss Jena GmbH (Germany)

Session Chairs

  • 1 Keynote Session

    • Ammasi Periasamy, University of Virginia (United States)

  • 2 Metabolism/NADH/FAD/Tryptophan I

    • Angelika C. Rueck, Universität Ulm (Germany)

  • 3 Metabolism/NADH/FAD/Tryptophan II

    • Elena V. Zagaynova, Nizhny Novgorod State Medical Academy (Russian Federation)

    • Marina V. Shirmanova, Nizhny Novgorod State Medical Academy (Russian Federation)

  • 4 Technology and In Vivo Imaging I

    • Peter T. C. So, Massachusetts Institute of Technology (United States)


    • Kevin W. Eliceiri, University of Wisconsin-Madison (United States)


    • Yuansheng Sun, ISS, Inc. (United States)

    • Margarida Barroso, Albany Medical College (United States)

  • 7 Second/Third Harmonic Generation

    • Paul J. Campagnola, University of Wisconsin-Madison (United States)

  • 8 Technology and In Vivo Imaging II

    • Fu-Jen Kao, National Yang-Ming University (Taiwan)

  • 9 Coherent Raman I

    • Ji-Xin Cheng, Purdue University (United States)

  • 10 Coherent Raman II

    • Wei Min, Columbia University (United States)

  • 11 Coherent Raman III

    • Lingyan Shi, Columbia University (United States)

  • 12 Technology and In Vivo Imaging III

    • Ammasi Periasamy, University of Virginia (United States)


Multiphoton microscopy has been established as the 3-D imaging method of choice for studying biomedical specimens from single cells and whole animals to patients with submicron resolution. 27 years have passed since the realization of two-photon laser scanning microscopy. The ever-expanding scope of applications and the continuing instrumental innovations requires a forum where new ideas can be exchanged and presented. Our conference at the SPIE BIOS 2018 meeting continues to address this need.

This is the 18th year of this conference and we start our conference with four Keynote lectures from leaders in the field of Metabolism:

Dr. Wei Min, Columbia University, NY, USA, “Seeing molecular vibrations: Chemical imaging for biomedicine.”

Dr. Enrico Gratton, University of California at Irvine, CA, USA, “Spectroscopic signatures of cells metabolism and extracellular species using phasor-FLIM.

Dr. Elena V. Zagaynova, Nizhny Novgorod State Medical Academy, Nizhny Novgorod, Russian Federation, “Metabolic imaging of tumor for diagnosis and response for therapy.

Dr. Lihong V. Wang, California Institute of Technology, CA, USA, “Photoacoustic tomography: Ultrasonically beating optical diffusion for deep imaging.

For 18 years in a row, the conference organized poster awards for the students and postdoctoral fellows. The poster award was donated by all the conference sponsors as acknowledged at the bottom of the page.

The 2 poster award winners are

  • 1. James R. W. Ulcickas, Purdue University, USA, “Mueller tensor approach for nonlinear optics in turbid media.” Paper 10498-109.

  • 2. Mengran Wang, Cornell University, USA, “Comparison of excitation wavelengths for in vivo deep imaging of mouse brain.” Paper 10498-110.

Some of the most valuable contributions in this volume are articles written by highly experienced practitioners of multiphoton microscopy. They have enumerated the most important considerations in designing multiphoton microscopes and imaging experiments. Further, updates on the state-of-the-art commercial multiphoton microscope systems are presented. This volume also includes articles describing some recent advances in major multiphoton microscope components and applications including laser light sources, ultrafast optics, filters, FRET, FLIM, FCS, Raman, CARS, SRS and Coherent Raman microscopy and spectroscopy, single molecule, endoscopy, In Vivo/Intravital imaging, metabolism measurements including NADH, FAD, tryptophan in cells and tissues and various scientific and clinical applications.

On a personal note, the conference chairs are grateful for the participation of all authors and session chairs. We acknowledge the innovation-driven manufacturers and sponsors of this conference [Applied Scientific Instrumentation, Becker & Hickl GmbH, Carl Zeiss, Chroma Technology Corp., Coherent Inc., ISS Inc., JenLab GmbH, Leica Microsystems, PicoQuant Photonics, Semrock Inc., and Spectra Physics & Newport (An MKS Company)] for their enthusiastic support in organizing this conference successfully for the last 18 years. We look forward to other exciting conferences in the future and welcome your continued participation and support.

Ammasi Periasamy

Peter T. C. So

Karsten König

Xiaoliang S. Xie

© (2018) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
"Front Matter: Volume 10498", Proc. SPIE 10498, Multiphoton Microscopy in the Biomedical Sciences XVIII, 1049801 (30 March 2018); doi: 10.1117/12.2323006; https://doi.org/10.1117/12.2323006

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