23 February 2018 Metabolic imaging of tumor for diagnosis and response for therapy
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Abstract
Nonlinear optical microscopy combined with fluorescence lifetime imaging is a non-invasive imaging technique, based on the study of fluorescence decay times of naturally occurring fluorescent molecules, enabling a noninvasive investigation of the biological tissue with subcellular resolution. Cancer exhibits altered cellular metabolism, which affects the autofluorescence of metabolic cofactors NAD(P)H and FAD. In this study features of tumor metabolism in different systems of organization (from cell culture to patient lesion) was showed. The observed differences in the relative contributions of free NAD(P)H and FAD testify to an increased a glycolytic metabolism in cancer cells compare to fibroblasts. In 3D spheroids, the cells of the proliferating zone had greater a1 and lower tm values than the cells of the quiescent zone, which likely is a consequence of their higher glycolytic rate. During the growth of colorectal cancer in the experimental mouse model, the contribution of the free component of NAD(P)H was increased. Dysplastic nevus and melanoma is characterized by raised contribution of free NADH compare to healthy skin. Therefore, melanoma cells had very short value of τ1.
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Elena Zagaynova, Elena Zagaynova, Marina Shirmanova, Marina Shirmanova, Maria Lukina, Maria Lukina, Varvara Dudenkova, Varvara Dudenkova, Nadezgda Ignatova, Nadezgda Ignatova, Vadim Elagin, Vadim Elagin, Irena Shlivko, Irena Shlivko, Vladislav Scheslavsky, Vladislav Scheslavsky, Natalia Orlinskay, Natalia Orlinskay, } "Metabolic imaging of tumor for diagnosis and response for therapy", Proc. SPIE 10498, Multiphoton Microscopy in the Biomedical Sciences XVIII, 1049804 (23 February 2018); doi: 10.1117/12.2295127; https://doi.org/10.1117/12.2295127
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