23 February 2018 Rapid in vivo vertical tissue sectioning by multiphoton tomography
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Abstract
A conventional tool in the pathological field is histology which involves the analysis of thin sections of tissue in which specific cellular structures are stained with different dyes. The process to obtain these stained tissue sections is time consuming and invasive as it requires tissue removal, fixation, sectioning, and staining. Moreover, imaging of live tissue is not possible. We demonstrate that multiphoton tomography can provide within seconds, non-invasive, label-free, vertical images of live tissue which are in quality similar to conventional light micrographs of histologic stained specimen. In contrast to conventional setups based on laser scanning which image horizontally sections, the vertical in vivo images are directly recorded by combined line scanning and timed adjustments of the height of the focusing optics. In addition, multiphoton tomography provides autofluorescence lifetimes which can be used to determine the metabolic states of cells.
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Ana Batista, Ana Batista, Hans Georg Breunig, Hans Georg Breunig, Karsten König, Karsten König, } "Rapid in vivo vertical tissue sectioning by multiphoton tomography", Proc. SPIE 10498, Multiphoton Microscopy in the Biomedical Sciences XVIII, 1049814 (23 February 2018); doi: 10.1117/12.2286915; https://doi.org/10.1117/12.2286915
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