CARS molecular fingerprinting using a sub-nanosecond supercontinuum light source

Hideaki Kano; Toshihiro Akiyama; Akihito Inoko; Tsubasa Kobayashi; Philippe Leproux; Vincent Couderc; Yuichi Kaji; Tetsuro Oshika

doi:10.1117/12.2287168

23 February 2018 CARS molecular fingerprinting using a sub-nanosecond supercontinuum light source

Hideaki Kano, Toshihiro Akiyama, Akihito Inoko, Tsubasa Kobayashi, Philippe Leproux, Vincent Couderc, Yuichi Kaji, Tetsuro Oshika

Author Affiliations +

Proceedings Volume 10498, Multiphoton Microscopy in the Biomedical Sciences XVIII; 104981P (2018) https://doi.org/10.1117/12.2287168
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

We have visualized living cells and tissues using an ultrabroadband multiplex coherent anti-Stokes Raman scattering (CARS) microspectroscopic system by using a sub-nanosecond supercontinuum (SC) light source. Owing to the ultrabroadband spectral profile of the SC, we can generate multiplex CARS signals in the spectral range of 500–3800 cm⁻¹, which covers the whole molecular fingerprint region, as well as the C-H and O-H stretching regions. Through the combination of the ultrabroadband multiplex CARS method with second harmonic generation (SHG) and third harmonic generation (THG) processes, we have successfully performed selective imaging of ciliary rootlet-composing Rootletin filaments in rat retina.

Conference Presentation

Citation Download Citation

Hideaki Kano, Toshihiro Akiyama, Akihito Inoko, Tsubasa Kobayashi, Philippe Leproux, Vincent Couderc, Yuichi Kaji, and Tetsuro Oshika "CARS molecular fingerprinting using a sub-nanosecond supercontinuum light source", Proc. SPIE 10498, Multiphoton Microscopy in the Biomedical Sciences XVIII, 104981P (23 February 2018); https://doi.org/10.1117/12.2287168

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available