23 February 2018 CARS molecular fingerprinting using a sub-nanosecond supercontinuum light source
Author Affiliations +
We have visualized living cells and tissues using an ultrabroadband multiplex coherent anti-Stokes Raman scattering (CARS) microspectroscopic system by using a sub-nanosecond supercontinuum (SC) light source. Owing to the ultrabroadband spectral profile of the SC, we can generate multiplex CARS signals in the spectral range of 500–3800 cm−1, which covers the whole molecular fingerprint region, as well as the C-H and O-H stretching regions. Through the combination of the ultrabroadband multiplex CARS method with second harmonic generation (SHG) and third harmonic generation (THG) processes, we have successfully performed selective imaging of ciliary rootlet-composing Rootletin filaments in rat retina.
Conference Presentation
© (2018) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Hideaki Kano, Hideaki Kano, Toshihiro Akiyama, Toshihiro Akiyama, Akihito Inoko, Akihito Inoko, Tsubasa Kobayashi, Tsubasa Kobayashi, Philippe Leproux, Philippe Leproux, Vincent Couderc, Vincent Couderc, Yuichi Kaji, Yuichi Kaji, Tetsuro Oshika, Tetsuro Oshika, } "CARS molecular fingerprinting using a sub-nanosecond supercontinuum light source", Proc. SPIE 10498, Multiphoton Microscopy in the Biomedical Sciences XVIII, 104981P (23 February 2018); doi: 10.1117/12.2287168; https://doi.org/10.1117/12.2287168

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