23 February 2018 Adaptive optics plug-and-play setup for high-resolution microscopes with multi-actuator adaptive lens
Author Affiliations +
Abstract
Adaptive Optics (AO) has revealed as a very promising technique for high-resolution microscopy, where the presence of optical aberrations can easily compromise the image quality. Typical AO systems however, are almost impossible to implement on commercial microscopes. We propose a simple approach by using a Multi-actuator Adaptive Lens (MAL) that can be inserted right after the objective and works in conjunction with an image optimization software allowing for a wavefront sensorless correction. We presented the results obtained on several commercial microscopes among which a confocal microscope, a fluorescence microscope, a light sheet microscope and a multiphoton microscope.
Conference Presentation
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M. Quintavalla, M. Quintavalla, P. Pozzi, P. Pozzi, Michelle Verhaegen, Michelle Verhaegen, Hielke Bijlsma, Hielke Bijlsma, Hans Verstraete, Hans Verstraete, S. Bonora, S. Bonora, } "Adaptive optics plug-and-play setup for high-resolution microscopes with multi-actuator adaptive lens", Proc. SPIE 10498, Multiphoton Microscopy in the Biomedical Sciences XVIII, 104981X (23 February 2018); doi: 10.1117/12.2290036; https://doi.org/10.1117/12.2290036
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