Herein, a method by which multiphoton microscopy is implemented to study a biofilm model of Staphylococcus epidermidis to noninvasively visualize and measure penetration of compounds in 3D biofilm structure and two photon excitation was exploited for spatially confined photoinactivation and microscopy optimized for evaluation of microbiological viability at a microscopic level. Future studies are aimed at future development of the proposed techniques for detailed studies of, e.g., quorum sensing and mechanisms contributing to antimicrobial resistance.
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Hanna Thomsen, Jeemol James, Anne Farewell, Marica B. Ericson, "Spatially confined photoinactivation of bacteria: towards novel tools for detailed mechanistic studies," Proc. SPIE 10498, Multiphoton Microscopy in the Biomedical Sciences XVIII, 1049825 (23 February 2018);