Characterization of a reflective objective with multiphoton microscopy

Mohammad M. Kabir; Aakash M. Choubal; Mayandi Sivaguru; Kimani C. Toussaint Jr.

doi:10.1117/12.2290305

23 February 2018 Characterization of a reflective objective with multiphoton microscopy

Mohammad M. Kabir, Aakash M. Choubal, Mayandi Sivaguru, Kimani C. Toussaint Jr.

Proceedings Volume 10498, Multiphoton Microscopy in the Biomedical Sciences XVIII; 104982Z (2018) https://doi.org/10.1117/12.2290305
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

Reflective objectives (ROs) can reduce chromatic aberration across a wide wavelength range in multiphoton microscopy (MPM). However, a systematic characterization of the performance of ROs has not been carried out. In this paper, we analyze the performance of a 0.5 numerical-aperture (NA) RO and compare it with a 0.55 NA standard glass objective (SO), using two-photon fluorescence (TPF) and second-harmonic generation (SHG). For experiments extending ~1 octave in visible and NIR wavelengths, the SO introduces defocusing errors of ~25% for TPF images of sub-diffraction fluorescent beads and ~10% for SHG images of collagen fibers. For both imaging systems, the RO provides a corresponding error of ~4%. This work highlights the potential usefulness of ROs for multimodal MPM applications.

Citation Download Citation

Mohammad M. Kabir, Aakash M. Choubal, Mayandi Sivaguru, and Kimani C. Toussaint Jr. "Characterization of a reflective objective with multiphoton microscopy", Proc. SPIE 10498, Multiphoton Microscopy in the Biomedical Sciences XVIII, 104982Z (23 February 2018); https://doi.org/10.1117/12.2290305

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
5 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Second-harmonic generation

Objectives

Multiphoton microscopy

Reflectivity

Microscopes

Microscopy

Point spread functions

Show All Keywords

Keywords/Phrases

Search In:

Publication Years