Recent advances in lasers for multiphoton microscopy (Conference Presentation)

Marco Arrigoni; Darryl McCoy

doi:10.1117/12.2291054

14 March 2018 Recent advances in lasers for multiphoton microscopy (Conference Presentation)

Marco Arrigoni, Darryl McCoy

Proceedings Volume 10498, Multiphoton Microscopy in the Biomedical Sciences XVIII; 104980J (2018) https://doi.org/10.1117/12.2291054
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

Multiphoton microscopy has become an ubiquitous research tool for neuroscience, disease studies, embryology and immunology. Refinements in non-linear imaging techniques have gone hand-in-hand with improvements in tuning range, power, pulse management and other features of femtosecond lasers specifically designed for these applications. While potential clinical applications require lasers with multiple outputs able to support multimodal imaging, recent developments in neuronal imaging like 3-photon microscopy or optogenetic activation require higher energies per pulse and longer wavelengths than conventional multiphoton microscopy. Here we describe the design and characteristics of laser sources able to address all these applications, staring with the more established ultrafast laser technology based on Titanium Sapphire lasers and their OPOs and covering recently developed sources like Ytterbium fiber mode-locked lasers and amplifiers with their tunable wavelength extensions.

Conference Presentation

Citation Download Citation

Marco Arrigoni and Darryl McCoy "Recent advances in lasers for multiphoton microscopy (Conference Presentation)", Proc. SPIE 10498, Multiphoton Microscopy in the Biomedical Sciences XVIII, 104980J (14 March 2018); https://doi.org/10.1117/12.2291054

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