23 February 2018 Particle tracking and extended object imaging by interferometric super resolution microscopy
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Abstract
An interferometric fluorescent microscope and a novel theoretic image reconstruction approach were developed and used to obtain super-resolution images of live biological samples and to enable dynamic real time tracking. The tracking utilizes the information stored in the interference pattern of both the illuminating incoherent light and the emitted light. By periodically shifting the interferometer phase and a phase retrieval algorithm we obtain information that allow localization with sub-2 nm axial resolution at 5 Hz.
Conference Presentation
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Itay Gdor, Seunghwan Yoo, Xiaolei Wang, Matthew Daddysman, Rosemarie Wilton, Nicola Ferrier, Mark Hereld, Oliver (Ollie) Cossairt, Aggelos Katsaggelos, Norbert F. Scherer, "Particle tracking and extended object imaging by interferometric super resolution microscopy", Proc. SPIE 10499, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXV, 1049911 (23 February 2018); doi: 10.1117/12.2291245; https://doi.org/10.1117/12.2291245
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