Imaging the developing heart: synchronized time-lapse microscopy during developmental changes

Carl J. Nelson; Charlotte Buckley; John J. Mullins; Martin A. Denvir; Jonathan Taylor

doi:10.1117/12.2290191

23 February 2018 Imaging the developing heart: synchronized time-lapse microscopy during developmental changes

Carl J. Nelson, Charlotte Buckley, John J. Mullins, Martin A. Denvir, Jonathan Taylor

Proceedings Volume 10499, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXV; 104991F (2018) https://doi.org/10.1117/12.2290191
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

How do you use imaging to analyse the development of the heart, which not only changes shape but also undergoes constant, high-speed, quasi-periodic changes? We have integrated ideas from prospective and retrospective optical gating to capture long-term, phase-locked developmental time-lapse videos. In this paper we demonstrate the success of this approach over a key developmental time period: heart looping, where large changes in heart shape prevent previous prospective gating approaches from capturing phase- locked videos. We use the comparison with other approaches to in vivo heart imaging to highlight the importance of collecting the most appropriate data for the biological question.

Citation Download Citation

Carl J. Nelson, Charlotte Buckley, John J. Mullins, Martin A. Denvir, and Jonathan Taylor "Imaging the developing heart: synchronized time-lapse microscopy during developmental changes", Proc. SPIE 10499, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXV, 104991F (23 February 2018); https://doi.org/10.1117/12.2290191

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