23 February 2018 Imaging the developing heart: synchronized time-lapse microscopy during developmental changes
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Abstract
How do you use imaging to analyse the development of the heart, which not only changes shape but also undergoes constant, high-speed, quasi-periodic changes? We have integrated ideas from prospective and retrospective optical gating to capture long-term, phase-locked developmental time-lapse videos. In this paper we demonstrate the success of this approach over a key developmental time period: heart looping, where large changes in heart shape prevent previous prospective gating approaches from capturing phase- locked videos. We use the comparison with other approaches to in vivo heart imaging to highlight the importance of collecting the most appropriate data for the biological question.
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Carl J. Nelson, Carl J. Nelson, Charlotte Buckley, Charlotte Buckley, John J. Mullins, John J. Mullins, Martin A. Denvir, Martin A. Denvir, Jonathan Taylor, Jonathan Taylor, } "Imaging the developing heart: synchronized time-lapse microscopy during developmental changes", Proc. SPIE 10499, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXV, 104991F (23 February 2018); doi: 10.1117/12.2290191; https://doi.org/10.1117/12.2290191
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