23 February 2018 Hyper-spectrum scanning laser optical tomography
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We describe a quantitative fluorescence projection tomography technique which measures the three-dimensional fluorescence spectrum in biomedical samples with size up to several millimeters. This is achieved by acquiring a series of hyperspectral images, by using laser scanning scheme, at different projection angles. We demonstrate that this technique provide a quantitative measure of the fluorescence signal by comparing the spectrum and intensity profile of a fluorescent bead phantom and also demonstrate its application to differentiating the extrinsic label and the autofluorescence in a mouse embryo.
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Lingling Chen, Guiye Li, Yingchao Li, Lina Liu, Ang Liu, Xuejuan Hu, Shuangchen Ruan, "Hyper-spectrum scanning laser optical tomography", Proc. SPIE 10499, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXV, 104991L (23 February 2018); doi: 10.1117/12.2287062; https://doi.org/10.1117/12.2287062

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