2 March 2018 Cross-level sampling for bandwidth effective time-domain fluorescence lifetime imaging implementation
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Abstract
The implementation of time domain fluorescence lifetime imaging (TD-FLIM) requires high cost bandwidth electronics and large storage capacity. A cross-level sampling technique for TD-FLIM is proposed. A simulation of this sampling technique was implemented using synthetic FLIM data. FLIM images were synthetically generated at different noise levels using a wide range of lifetimes. Each of the images was resampled using a cross-level approach and the lifetime maps were computed. Simulation results displayed strong correlation between the lifetime maps of the original and resampled images, suggesting that this sampling method could be adopted to reduce bandwidth and data transfer/ storage requirements.
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Elvis Duran, Elvis Duran, Sebastian Hoyos, Sebastian Hoyos, Javier Jo, Javier Jo, } "Cross-level sampling for bandwidth effective time-domain fluorescence lifetime imaging implementation", Proc. SPIE 10499, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXV, 104991N (2 March 2018); doi: 10.1117/12.2287478; https://doi.org/10.1117/12.2287478
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