Round robin test on V-shape bio-imaging transfer standard for determination of the instrument transfer function of 3D optical profilers

Carlos Bermudez; Roger Artigas; Pol Martinez; Anton Nolvi; Miikka Järvinen; Edward Hæggström; Ivan Kassamakov

doi:10.1117/12.2289098

23 February 2018 Round robin test on V-shape bio-imaging transfer standard for determination of the instrument transfer function of 3D optical profilers

Carlos Bermudez, Roger Artigas, Pol Martinez, Anton Nolvi, Miikka Järvinen, Edward Hæggström, Ivan Kassamakov

Author Affiliations +

Proceedings Volume 10499, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXV; 1049923 (2018) https://doi.org/10.1117/12.2289098
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

A V-shape Bio-Transfer-Standard (V-BTS), developed and produced at the University of Helsinki (UH), was measured in two laboratories. In comparison to Siemens Star calibration specimens, the V-BTS performs better at high lateral frequencies close to the diffraction limit of the optical instrument. This permits determining of the Instrument Transfer Function (ITF). The V-BTS features two lipid bilayer steps that partly overlap each other at an angle of 20°, with an average height of 4.6 ± 0.1 nm. The Round Robin (RR) test aims to determine whether the V-BTS and the developed application protocol work with different optical profilers in different laboratories. First the artefact was measured at Sensofar-Tech, S.L. using an S-neox profiler working in Phase Shifting Interferometry mode. Then V-BTS was measured at UH using a custom-built Scanning White Light Interferometer. All measurements done by four different operators at the two laboratories have a range or standard deviation of ±0.1 nm which agrees with the theoretical estimates and with measurements done using an atomic force microscope and with a surface plasmon resonance based instrument. The RR results show the applicability of the V-BTS for calibration and for ITF characterization of 3D optical profilers.

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Carlos Bermudez, Roger Artigas, Pol Martinez, Anton Nolvi, Miikka Järvinen, Edward Hæggström, and Ivan Kassamakov "Round robin test on V-shape bio-imaging transfer standard for determination of the instrument transfer function of 3D optical profilers", Proc. SPIE 10499, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXV, 1049923 (23 February 2018); https://doi.org/10.1117/12.2289098

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