20 February 2018 Measuring 3D molecular orientation and rotational mobility using a Tri-spot point spread function
Author Affiliations +
Abstract
We present a method to measure the molecular orientation and rotational mobility of single-molecule emitters by designing and implementing a Tri-spot point spread function. It can measure all degrees of freedom related to molecular orientation and rotational mobility. Its design is optimized by maximizing the theoretical limit of its measurement precision. We evaluate the precision and accuracy of the Tri-spot PSF by measuring the orientation and effective rotational mobility of single fluorescent molecules embedded in a polymer matrix.
Conference Presentation
© (2018) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Oumeng Zhang, Oumeng Zhang, Tianben Ding, Tianben Ding, Jin Lu, Jin Lu, Hesam Mazidi, Hesam Mazidi, Matthew D. Lew, Matthew D. Lew, } "Measuring 3D molecular orientation and rotational mobility using a Tri-spot point spread function", Proc. SPIE 10500, Single Molecule Spectroscopy and Superresolution Imaging XI, 105000B (20 February 2018); doi: 10.1117/12.2287004; https://doi.org/10.1117/12.2287004
PROCEEDINGS
11 PAGES + PRESENTATION

SHARE
Back to Top