20 February 2018 A simple and low-cost structured illumination microscopy using a pico-projector
Author Affiliations +
Abstract
Here, development of a low-cost structured illumination microscopy (SIM) based on a pico-projector is presented. The pico-projector consists of independent red, green and blue LEDs that remove need for an external illumination source. Moreover, display element of the pico-projector serves as a pattern generating spatial light modulator. A simple lens group is employed to couple light from the projector to an epi-illumination port of a commercial microscope system. 2D sub SIM images are acquired and synthesized to surpass the diffraction limit using 40x (0.75 NA) objective. Resolution of the reconstructed SIM images is verified with a dye-and-object object and a fixed cell sample.
Conference Presentation
© (2018) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Baturay Özgürün, Baturay Özgürün, } "A simple and low-cost structured illumination microscopy using a pico-projector", Proc. SPIE 10500, Single Molecule Spectroscopy and Superresolution Imaging XI, 105000C (20 February 2018); doi: 10.1117/12.2286790; https://doi.org/10.1117/12.2286790
PROCEEDINGS
6 PAGES + PRESENTATION

SHARE
RELATED CONTENT

Quantitative phase imaging with programmable illumination
Proceedings of SPIE (March 10 2015)
Point spread function engineering with multiphoton SPIFI
Proceedings of SPIE (March 08 2016)
Advanced SLMs for microscopy
Proceedings of SPIE (February 22 2018)
Light-sheet optimization for microscopy
Proceedings of SPIE (March 14 2016)
SLM-based microscopy
Proceedings of SPIE (May 04 2012)

Back to Top