20 February 2018 Superresolution fluorescence imaging by pump-probe setup using repetitive stimulated transition process
Author Affiliations +
Abstract
We proposed superresolution nonlinear fluorescence microscopy with pump-probe setup that utilizes repetitive stimulated absorption and stimulated emission caused by two-color laser beams. The resulting nonlinear fluorescence that undergoes such a repetitive stimulated transition is detectable as a signal via the lock-in technique. As the nonlinear fluorescence signal is produced by the multi-ply combination of incident beams, the optical resolution can be improved. A theoretical model of the nonlinear optical process is provided using rate equations, which offers phenomenological interpretation of nonlinear fluorescence and estimation of the signal properties. The proposed method is demonstrated as having the scalability of optical resolution. Theoretical resolution and bead image are also estimated to validate the experimental result.
Conference Presentation
© (2018) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Fumihiro Dake, Fumihiro Dake, Naoki Fukutake, Naoki Fukutake, Seri Hayashi, Seri Hayashi, Yusuke Taki, Yusuke Taki, } "Superresolution fluorescence imaging by pump-probe setup using repetitive stimulated transition process", Proc. SPIE 10500, Single Molecule Spectroscopy and Superresolution Imaging XI, 105000Q (20 February 2018); doi: 10.1117/12.2287325; https://doi.org/10.1117/12.2287325
PROCEEDINGS
6 PAGES + PRESENTATION

SHARE
RELATED CONTENT


Back to Top