20 February 2018 A simple and low-cost structured illumination microscopy using a pico-projector
Author Affiliations +
Here, development of a low-cost structured illumination microscopy (SIM) based on a pico-projector is presented. The pico-projector consists of independent red, green and blue LEDs that remove need for an external illumination source. Moreover, display element of the pico-projector serves as a pattern generating spatial light modulator. A simple lens group is employed to couple light from the projector to an epi-illumination port of a commercial microscope system. 2D sub SIM images are acquired and synthesized to surpass the diffraction limit using 40x (0.75 NA) objective. Resolution of the reconstructed SIM images is verified with a dye-and-object object and a fixed cell sample.
Conference Presentation
© (2018) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Baturay Özgürün, Baturay Özgürün, } "A simple and low-cost structured illumination microscopy using a pico-projector", Proc. SPIE 10500, Single Molecule Spectroscopy and Superresolution Imaging XI, 105000C (20 February 2018); doi: 10.1117/12.2286790; https://doi.org/10.1117/12.2286790

Back to Top