Aberrations can be suppressed by implementing sensorless adaptive optics techniques, whereby aberration correction is achieved by maximising a certain image quality metric. In confocal microscopy for example, one can employ the total image brightness as an image quality metric. Aberration correction is subsequently achieved by iteratively changing the settings of a wavefront corrector device until the metric is maximised. This simplistic approach has limited applicability to isoSTED microscopy where, due to the complex interplay between the excitation and depletion foci, maximising the total image brightness can lead to introducing aberrations in the depletion foci. In this work we first consider the effects that different aberration modes have on isoSTED microscopes. We then propose an iterative, wavelet-based aberration correction algorithm and evaluate its benefits.
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Jacopo Antonello, Xiang Hao, Edward S. Allgeyer, Joerg Bewersdorf, Jens Rittscher, Martin J. Booth, "Sensorless adaptive optics for isoSTED nanoscopy," Proc. SPIE 10502, Adaptive Optics and Wavefront Control for Biological Systems IV, 1050206 (23 February 2018);