23 February 2018 Practical guidelines for implementing adaptive optics in fluorescence microscopy
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Abstract
In life sciences, interest in the microscopic imaging of increasingly complex three dimensional samples, such as cell spheroids, zebrafish embryos, and in vivo applications in small animals, is growing quickly. Due to the increasing complexity of samples, more and more life scientists are considering the implementation of adaptive optics in their experimental setups. While several approaches to adaptive optics in microscopy have been reported, it is often difficult and confusing for the microscopist to choose from the array of techniques and equipment. In this poster presentation we offer a small guide to adaptive optics providing general guidelines for successful adaptive optics implementation.
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Dean Wilding, Dean Wilding, Paolo Pozzi, Paolo Pozzi, Oleg Soloviev, Oleg Soloviev, Gleb Vdovin, Gleb Vdovin, Michel Verhaegen, Michel Verhaegen, } "Practical guidelines for implementing adaptive optics in fluorescence microscopy", Proc. SPIE 10502, Adaptive Optics and Wavefront Control for Biological Systems IV, 105021F (23 February 2018); doi: 10.1117/12.2287647; https://doi.org/10.1117/12.2287647
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