23 February 2018 Multi-spectral digital holographic microscopy for enhanced quantitative phase imaging of living cells
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Proceedings Volume 10503, Quantitative Phase Imaging IV; 1050313 (2018) https://doi.org/10.1117/12.2291071
Event: SPIE BiOS, 2018, San Francisco, California, United States
Abstract
Main restrictions of using laser light in digital holographic microscopy (DHM) are coherence induced noise and parasitic reflections in the experimental setup which limit resolution and measurement accuracy. We explored, if coherence properties of partial coherent light sources can be generated synthetically utilizing spectrally tunable lasers. The concept of the method is demonstrated by label-free quantitative phase imaging of living pancreatic tumor cells and utilizing an experimental configuration including a commercial microscope and a laser source with a broad tunable spectral range of more than 200 nm.
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Björn Kemper, Lena Kastl, Jürgen Schnekenburger, Steffi Ketelhut, "Multi-spectral digital holographic microscopy for enhanced quantitative phase imaging of living cells", Proc. SPIE 10503, Quantitative Phase Imaging IV, 1050313 (23 February 2018); doi: 10.1117/12.2291071; https://doi.org/10.1117/12.2291071
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