15 March 2018 Assessing tissues' inhomogeneous optical properties based on quantitative phase imaging and dark field microscopy with LED illumination (Conference Presentation)
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Proceedings Volume 10503, Quantitative Phase Imaging IV; 1050315 (2018) https://doi.org/10.1117/12.2293608
Event: SPIE BiOS, 2018, San Francisco, California, United States
Abstract
Assessing tissues’ inhomogeneous optical properties is helpful for label-free diagnosis. In this paper, a method and a setup are proposed to assess the tissues’ inhomogeneous optical properties based on quantitative phase imaging (QPI) and dark-field microscopy using LED illumination. In the bright-field and coherent dark-field imaging, quantitative phase (QP) can be recovered from multi-frame intensity images captured at different axial positions by Transport-intensity-equation (TIE) and multi-height Gerchberg-Saxton(G-S) phase retrieval algorithms. In the incoherent dark-field imaging, some scattering samples can be observed easily without any computation and the resolution can be improved in contrast to bright-field illumination. According to the scattering-phase theorem and the statistical dispersion relation (SDR), tissues’ inhomogeneous optical properties can be assessed. In our experiments, we find, it is a good idea to combine QP maps and dark-field images to assess inhomogeneous optical properties of bio-samples. In contrast to the DHM and interferometers, our setup is cost-effective and use-flexible, thus having the potential to serve as a very powerful tool in biological applications.
Conference Presentation
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Qiulan Liu, Qiulan Liu, Cuifang Kuang, Cuifang Kuang, Xu Liu, Xu Liu, } "Assessing tissues' inhomogeneous optical properties based on quantitative phase imaging and dark field microscopy with LED illumination (Conference Presentation)", Proc. SPIE 10503, Quantitative Phase Imaging IV, 1050315 (15 March 2018); doi: 10.1117/12.2293608; https://doi.org/10.1117/12.2293608
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