23 February 2018 Phase from defocus
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Proceedings Volume 10503, Quantitative Phase Imaging IV; 105031Q (2018) https://doi.org/10.1117/12.2287693
Event: SPIE BiOS, 2018, San Francisco, California, United States
Abstract
We present a simple and compact phase imaging microscope for long-term observation of non-absorbing biological samples such as unstained cells in nutritive media. The phase image is obtained from a single defocused image taken with a standard wide-field microscope. Using a semi-coherent light source allows us to computationally re-focus image post-acquisition and recover both phase and transmission of the complex specimen. The simplicity of the system reduces both the cost and its physical size and allows a long-term observation of samples directly in a standard biological incubator. The low cost of the system can contribute to the democratization of science by allowing to perform complex long-term biological experiments to the laboratories with constrained budget. In this proceeding we present several results taken with our prototype and discuss the possibilities and limitations of our system.
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Ondrej Mandula, Cédric Allier, Lionel Hervé, Eric Denarier, Anne Fourest-Lieuvin, Sylvie Gory-Fauré, Angélique Vinit, Sophie Morales, "Phase from defocus", Proc. SPIE 10503, Quantitative Phase Imaging IV, 105031Q (23 February 2018); doi: 10.1117/12.2287693; https://doi.org/10.1117/12.2287693
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