Quantitative phase imaging for enhanced assessment of optomechanical cancer cell properties

Lena Kastl; Björn Kemper; Jürgen Schnekenburger

doi:10.1117/12.2290970

23 February 2018 Quantitative phase imaging for enhanced assessment of optomechanical cancer cell properties

Lena Kastl, Björn Kemper, Jürgen Schnekenburger

Proceedings Volume 10503, Quantitative Phase Imaging IV; 105032R (2018) https://doi.org/10.1117/12.2290970
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

Optical cell stretching provides label-free investigations of cells by measuring their biomechanical properties based on deformability determination in a fiber optical two-beam trap. However, the stretching forces in this two-beam laser trap depend on the optical properties of the investigated specimen. Therefore, we characterized in parallel four cancer cell lines with varying degree of differentiation utilizing quantitative phase imaging (QPI) and optical cell stretching. The QPI data allowed enhanced assessment of the mechanical cell properties measured with the optical cell stretcher and demonstrates the high potential of cell phenotyping when both techniques are combined.

Citation Download Citation

Lena Kastl, Björn Kemper, and Jürgen Schnekenburger "Quantitative phase imaging for enhanced assessment of optomechanical cancer cell properties", Proc. SPIE 10503, Quantitative Phase Imaging IV, 105032R (23 February 2018); https://doi.org/10.1117/12.2290970

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