25 April 2018 Reconstruction distance formula for Off-axis SIDH
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Proceedings Volume 10503, Quantitative Phase Imaging IV; 105032U (2018) https://doi.org/10.1117/12.2289927
Event: SPIE BiOS, 2018, San Francisco, California, United States
Abstract
Even though holography is a powerful technique for three-dimensional (3D) imaging, it could not be applied easily to an incoherent imaging system, for instance fluorescence microscopy. 3D imaging capability in fluorescence microscopy becomes more and more important lately because of great research demand on cell behavior changes in 3D environmental conditions triggered by recently developed 3D cell culture techniques. Biological research on contact-based cell-to-cell or membrane-to-membrane interactions need 3D imaging as well. Lately, several incoherent holographic imaging methods such as scanning holography, self-interference incoherent digital holography (SIDH), and Fresnel incoherent correlation holography (FINCH) have been proposed for 3D holographic imaging with incoherent light. Most of these methods commonly use an in-line holographic microscopy configuration, which has fundamental image quality degradation problems associated with the two DC terms and the twin image term in a measured hologram. It can be removed by taking multiple holograms with different phase delays in measurement or by iterative methods in numerical reconstruction algorithms. Even though off-axis holography is a straightforward method to remove the DC terms and the twin image, it is difficult to be used with incoherent light due to large path length difference between two beams in an off-axis holographic microscopy configuration. In this study we propose a new off-axis FINCH system which can overcome the path length mismatch problem by using a wedge plate. The combination of a spatial light modulator and a wedge plate in our proposed off-axis FINCH system makes the path lengths of two beams almost identical.
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Philjun Jeon, Heejung Lee, Jongwu Kim, Dugyoung Kim, "Reconstruction distance formula for Off-axis SIDH", Proc. SPIE 10503, Quantitative Phase Imaging IV, 105032U (25 April 2018); doi: 10.1117/12.2289927; https://doi.org/10.1117/12.2289927
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