20 February 2018 Detection and identification of amino acids in Ficoll solutions with femtosecond laser-induced breakdown spectroscopy
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Abstract
We report femtosecond Laser-induced Breakdown Spectroscopy (fs-LIBS) measurements on several amino acids (Serine, Glutamine, and Cysteine) and Albumin protein solutions mixed with Ficoll polysaccharide at different proportions. The goal is to assess the effects of a host matrix on the identification and spectral characterization of amino acids by fs-LIBS. fs-LIBS utilizes an intense short laser pulse to obliterate a sample into basic constituents and to record the emission spectrum of atoms, ions, and molecules in the cooling down of the plasma plume. Several spectral peaks associated primarily with elemental composition of a sample were observed in the fs-LIBS spectra in a range from 200 to 950 nm. In addition, some molecular information associated with diatomic vibrational modes in certain molecules such as C-C and C-N were also obtained. The presence of Ficoll affects the relative intensity and broadening of the CN band, which could be considered as signatures of the amino acids. The fs-LIBS data and their analysis compare favorably with those derived from Fourier Transform Infrared Spectroscopy (FTIR). Interpretation of the spectral information enclosed in the emission of the diatomic molecules during laser ablation may lead to a better understanding of plume chemistry with a direct consequence on chemical analysis of complex samples such as amino acids. Altogether, the results demonstrate the potential of fs-LIBS technique as a detection method of biomolecules and for probing interactions of these biomolecules with a host matrix.
Conference Presentation
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Y. Markushin, Y. Markushin, P. Sivakumar, P. Sivakumar, N. Melikechi, N. Melikechi, H. Boukari, H. Boukari, } "Detection and identification of amino acids in Ficoll solutions with femtosecond laser-induced breakdown spectroscopy", Proc. SPIE 10504, Biophysics, Biology and Biophotonics III: the Crossroads, 1050409 (20 February 2018); doi: 10.1117/12.2290611; https://doi.org/10.1117/12.2290611
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