15 March 2018 Stimulated Raman scattering microscopy and spectroscopy with a rapidly scanning optical delay line (Conference Presentation)
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Abstract
Stimulated Raman scattering (SRS) microscopy that is capable of both high speed imaging and rapid spectroscopy will be advantageous for detailed chemical analysis of heterogeneous biological specimens. We have developed a system based on spectral focusing SRS technology, with the integration of a rapid scanning optical delay line (RSODL), which allows continuous tuning of SRS spectra by scanning a galvo mirror. We demonstrated SRS spectral measurements of dimethyl sulfoxide solution at low concentrations, and multi-color imaging of rice pollens and HeLa cells with line-by-line delay tuning to reduce motion artifacts, as well as fast acquisition of SRS spectra at specific regions of interest.
Conference Presentation
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Minbiao Ji, "Stimulated Raman scattering microscopy and spectroscopy with a rapidly scanning optical delay line (Conference Presentation)", Proc. SPIE 10505, High-Speed Biomedical Imaging and Spectroscopy III: Toward Big Data Instrumentation and Management, 105050M (15 March 2018); doi: 10.1117/12.2292948; https://doi.org/10.1117/12.2292948
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