20 February 2018 Ultrasensitive colocalization detection based on plasmonic nanolithography with molecular-overlapped optical near-fields
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Abstract
The detection sensitivity of surface plasmon resonance (SPR) biosensors has been improved by employing colocalization of spatial distribution of electromagnetic near-fields and detection molecules. We have used plasmon nanolithography to achieve light-matter colocalization on triangular nanoaperture arrays and optimized array configurations to improve colocalization efficiency. Streptavidin-biotin interactions were measured to validate the concept. It was confirmed that colocalized distributions of target binding and localized near-fields produced larger optical detection sensitivity. The colocalized detection was also shown to come with wider dynamic range than noncolocalized detection. The effective limit-of-detection of colocalized measurements was on the order of 30 pM. The colocalized detection sensitivity was estimated to be below 1 fg/mm2 in a 100-nm deep evanescent area, an enhancement by more than three orders of magnitude over conventional SPR sensor.
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Kiheung Kim, Wonju Lee, Kyungwha Chung, Hongki Lee, Taehwang Son, Youngjin Oh, Yun-Feng Xiao, Dong Ha Kim, Donghyun Kim, "Ultrasensitive colocalization detection based on plasmonic nanolithography with molecular-overlapped optical near-fields", Proc. SPIE 10506, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XV, 1050610 (20 February 2018); doi: 10.1117/12.2291449; https://doi.org/10.1117/12.2291449
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