Remote enzyme activation using gold coated magnetite as antennae for radio frequency fields

Christian B. Collins; Christopher J. Ackerson

doi:10.1117/12.2290478

23 February 2018 Remote enzyme activation using gold coated magnetite as antennae for radio frequency fields

Christian B. Collins, Christopher J. Ackerson

Proceedings Volume 10507, Colloidal Nanoparticles for Biomedical Applications XIII; 105070F (2018) https://doi.org/10.1117/12.2290478
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

The emerging field of remote enzyme activation, or the ability to remotely turn thermophilic increase enzyme activity, could be a valuable tool for understanding cellular processes. Through exploitation of the temperature dependence of enzymatic processes and high thermal stability of thermophilic enzymes these experiments utilize nanoparticles as ‘antennae’ that convert radiofrequency (RF) radiation into local heat, increasing activity of the enzymes without increasing the temperature of the surrounding bulk solution. To investigate this possible tool, thermolysin, a metalloprotease was covalently conjugated to 4nm gold coated magnetite particles via peptide bond formation with the protecting ligand shell. RF stimulated protease activity at 17.76 MHz in a solenoid shaped antenna, utilizing both electric and magnetic field interactions was investigated. On average 40 percent higher protease activity was observed in the radio frequency fields then when bulk heating the sample to the same temperature. This is attributed to electrophoretic motion of the nanoparticle enzyme conjugates and local regions of heat generated by the relaxation of the magnetite cores with the oscillating field. Radio frequency local heating of nanoparticles conjugated to enzymes as demonstrated could be useful in the activation of specific enzymes in complex cellular environments.

Citation Download Citation

Christian B. Collins and Christopher J. Ackerson "Remote enzyme activation using gold coated magnetite as antennae for radio frequency fields", Proc. SPIE 10507, Colloidal Nanoparticles for Biomedical Applications XIII, 105070F (23 February 2018); https://doi.org/10.1117/12.2290478

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available