17 May 1989 Distances And Distance Distributions In A Ca2+-Binding Protein
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Proceedings Volume 1054, Fluorescence Detection III; (1989) https://doi.org/10.1117/12.951554
Event: OE/LASE '89, 1989, Los Angeles, CA, United States
If a distribution of separations exists between a fluorescent donor group and an absorbing acceptor group, then the apparent efficiency of radiationless energy transfer will be different when monitored by measurements of static fluorescence intensity or average fluorescence decay time. This may be used to compute the parameters characterizing the distribution of separations, assuming it is Gaussian in form. Parameters computed in this way agree with those obtained by the frequency domain method for model systems. The distribution of separations has been computed for a donor group linked to Met-25 of troponin C and an acceptor group linked to Cys-98. The distribution narrows in the presence of Ca2+.
© (1989) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Robert F. Steiner, Robert F. Steiner, Jianqing Lan, Jianqing Lan, Louise Garone, Louise Garone, Sharon Albaugh, Sharon Albaugh, } "Distances And Distance Distributions In A Ca2+-Binding Protein", Proc. SPIE 1054, Fluorescence Detection III, (17 May 1989); doi: 10.1117/12.951554; https://doi.org/10.1117/12.951554

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