22 February 2018 Use of digital micromirror devices as dynamic pinhole arrays for adaptive confocal fluorescence microscopy
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Abstract
In this work, we present a new confocal laser scanning microscope capable to perform sensorless wavefront optimization in real time. The device is a parallelized laser scanning microscope in which the excitation light is structured in a lattice of spots by a spatial light modulator, while a deformable mirror provides aberration correction and scanning. A binary DMD is positioned in an image plane of the detection optical path, acting as a dynamic array of reflective confocal pinholes, images by a high performance cmos camera. A second camera detects images of the light rejected by the pinholes for sensorless aberration correction.
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Paolo Pozzi, Paolo Pozzi, Dean Wilding, Dean Wilding, Oleg Soloviev, Oleg Soloviev, Gleb Vdovin, Gleb Vdovin, Michel Verhaegen, Michel Verhaegen, } "Use of digital micromirror devices as dynamic pinhole arrays for adaptive confocal fluorescence microscopy", Proc. SPIE 10546, Emerging Digital Micromirror Device Based Systems and Applications X, 105460D (22 February 2018); doi: 10.1117/12.2288436; https://doi.org/10.1117/12.2288436
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