We developed a custom automated SPLM imaging system based on an upright microscope and a digital micromirror device (DMD) projector. The high spatial frequency patterns were used to achieve effective background suppression. Enhanced scattering sensitivity with SPLM resulted in images with highly improved visibility of collagen structures, even of tissues covered by pigment. SPLM produced improved fiber orientation maps from superficial layers compared to depth-averaged orientation from regular PLM. SPLM imaging provides valuable information of collagen fiber morphology and orientation in situ thus strengthening the study of ocular collagen fiber biomechanics and glaucoma.
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Bin Yang, Bryn Brazile, Ning-Jiun Jan, Andrew P. Voorhees, Ian A. Sigal, "Structured polarized light microscopy (SPLM) for mapping collagen fiber orientation of ocular tissues ," Proc. SPIE 10546, Emerging Digital Micromirror Device Based Systems and Applications X, 105460I (22 February 2018);