Delivery of nanoparticles inside the cytosol of live cells for the monitoring of cellular processes (Conference Presentation)

Xizhen Lian; Alfredo Erazo-Oliveras; Hong-Cai Zhou; Jean-Philippe Pellois

doi:10.1117/12.2300544

14 March 2018 Delivery of nanoparticles inside the cytosol of live cells for the monitoring of cellular processes (Conference Presentation)

Xizhen Lian, Alfredo Erazo-Oliveras, Hong-Cai Zhou, Jean-Philippe Pellois

Author Affiliations +

Proceedings Volume 10547, Advances in Photonics of Quantum Computing, Memory, and Communication XI; 1054703 (2018) https://doi.org/10.1117/12.2300544
Event: SPIE OPTO, 2018, San Francisco, California, United States

Abstract

The delivery of MOF-enzyme nanofactories (50-100 nm in diameter) into the cytosol of cells will be presented. Delivery is achieved by co-incubation with the peptide dfTAT, a dimeric construct of the HIV TAT peptide. Cytosolic targeting is highly efficient and dfTAT circumvents the endosomal entrapment of macromolecular cargos. The delivery protocol does not require interactions between dfTAT and nanoparticles, thereby eliminating complex labeling steps. Cytosolic access is rapid and cells recover from the delivery process in a matter of minutes. Overall, this approach should facilitate the monitoring of intracellular processes by nanoparticle probes.

Conference Presentation

Citation Download Citation

Xizhen Lian, Alfredo Erazo-Oliveras, Hong-Cai Zhou, and Jean-Philippe Pellois "Delivery of nanoparticles inside the cytosol of live cells for the monitoring of cellular processes (Conference Presentation)", Proc. SPIE 10547, Advances in Photonics of Quantum Computing, Memory, and Communication XI, 1054703 (14 March 2018); https://doi.org/10.1117/12.2300544

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