Cytochrome C Oxidase: The Influence Of The "Open-Closed" Transition On Cytochrome A[sub]3[/sub]

Denis L. Rousseau; Yuan-chin Ching; Sanghwa Han; Massimo Sassaroli; Satish Singh

doi:10.1117/12.951595

5 July 1989 Cytochrome C Oxidase: The Influence Of The "Open-Closed" Transition On Cytochrome A₃

Denis L. Rousseau; Yuan-chin Ching; Sanghwa Han; Massimo Sassaroli; Satish Singh

Author Affiliations +

Proceedings Volume 1055, Raman Scattering, Luminescence and Spectroscopic Instrumentation in Technology; (1989) https://doi.org/10.1117/12.951595
Event: OE/LASE '89, 1989, Los Angeles, CA, United States

Abstract

Cytochrome c oxidase is known to display a conformational transition from a "closed" to an "open" structure upon partial reduction. In the past exogenous ligand binding kinetic differences and differences in tryptophan fluorescence have been associated with this structural change. We report changes in the resonance Raman spectrum of NO-bound cytochrome as upon this transition. We detect changes in both the heme vibrational modes and in the Fe-N-0 bending mode. Furthermore, we have been able to identify a hydroxide-bound intermediate in the enzyme. Analysis of the conditions for its generation lead to the proposal that it is stable only in the open conformation of the protein. In both of these series of experiments advantage was taken of the photoreducibility of the heme groups by the incident laser beam.

Citation Download Citation

Denis L. Rousseau, Denis L. Rousseau, Yuan-chin Ching, Yuan-chin Ching, Sanghwa Han, Sanghwa Han, Massimo Sassaroli, Massimo Sassaroli, Satish Singh, Satish Singh, } "Cytochrome C Oxidase: The Influence Of The "Open-Closed" Transition On Cytochrome A₃", Proc. SPIE 1055, Raman Scattering, Luminescence and Spectroscopic Instrumentation in Technology, (5 July 1989); doi: 10.1117/12.951595; https://doi.org/10.1117/12.951595

ACCESS THE FULL ARTICLE

PERSONAL SIGN IN
Full access may be available with your subscription

Email or Username Forgot your username?

Password Forgot your password?

Show

Remember Email/Username on this computer

Remember Password

No SPIE account? Create an account
or Institutional Sign In via Shibboleth

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $14.40

Non-members: $18.00 ADD TO CART

Show All Keywords

Keywords/Phrases

Search In:

Publication Years