12 March 2018 Uptake of L-maurocalcine in DAOY cells and bio-distribution in mice by SPECT/CT imaging
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Abstract
Background: Cell permeable peptides (CPP) are a new class of carrier molecule to deliver biomolecules, radio-nucleotide and drugs that is gaining momentum. CPP are capable of entering into the cells by breaking the resistance of the membrane barrier and thus can be used universally in many cell types, which renders it an efficient carrier for both in-vitro and in-vivo use.

Methods: L-Maurocalcine (L-MCa), a peptide derived from scorpion venom was radiolabeled with 125I using the lactoperoxidase method. We achieved a specific activity of 45Mbq/nmole. In vitro studies with 125I-L-MCa in DAOY cells (human medulloblastoma) were studied in order to analyze the uptake of the peptide. 125I-L-MCa was injected intravenously in mice through tail vein and bio-distribution was studied using single photon emission tomography/computed tomography (SPECT/CT).

Results: The cellular uptake of the 125I-L-MCa in DAOY cells was time and dose dependent suggesting that the radiolabeled peptide retains the biological property after radiolabeling. We have observed no loss of cell viability upon uptake of 125I-L-MCa, favoring that this peptide has potential for use in in vivo studies. The distribution of the 125I-L-MCa in mice revealed its uptake in the liver, kidney and stomach. Interestingly the 125I-L-MCa was cleared from the circulation 24h post injection, thus providing another advantage for its use in in vivo studies.

Conclusions: In the present study we have shown the uptake of 125I-L-MCa in DAOY cells. Further, the 125I-L-MCa when injected in mice localized to the liver, kidney and stomach as revealed by SPECT/CT. Cells labeled with 125I-L-MCa can possibly be tracked to their target site.
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Poonam Sarkar, Poonam Sarkar, M. Waleed Gaber, M. Waleed Gaber, "Uptake of L-maurocalcine in DAOY cells and bio-distribution in mice by SPECT/CT imaging", Proc. SPIE 10578, Medical Imaging 2018: Biomedical Applications in Molecular, Structural, and Functional Imaging, 105782F (12 March 2018); doi: 10.1117/12.2293973; https://doi.org/10.1117/12.2293973
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