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12 April 2018 The effect of phototherapies on bone repair of euthyroid and hypothyroid rats: Raman spectroscopic study
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Abstract
The repair of bone tissue is complex and can be influenced by several local and systemic factors that can delay the repair. Laser and LED phototherapies have shown positive results in the repair of bone tissue. The aim of this study was to evaluate, through Raman spectroscopy, the influence of laser (λ780 nm) and LED (λ850 nm) phototherapies in the repair of surgical defects in femurs of euthyroid and hypothyroid rats. Thirty Albinus Wistar rats were randomly divided into 6 groups. The animals of the hypothyroid groups were submitted to surgical removal of the thyroid gland. After general anesthesia, a surgical bone defect was created in the femur of each animal and filled with blood clot in all groups. In group I (Euthyroid) the defect was created in euthyroid animals; In Group II (Hypo) the defect was created in hypothyroid animals; In Group III (Euthyroid Laser) the defect was irradiated with Laser; on Group IV (Hypo Laser) the defect was made in a hypothyroid animal and irradiated with Laser; on Group V (Euthyroid LED) the defect was irradiated with LED and on Group VI (Hypo LED) the defect was created in hypothyroid animals and LED irradiated. Irradiation was carried out at every 48-h for 15 days. Specimens were taken and stored in liquid nitrogen. Intensity of peaks of phosphate HA (~960 cm-1), carbonated HA (~1,070 cm-1) and collagen (~1,414 cm-1) were measured by Raman Spectroscopy. The results showed that the health status had significant influence all peaks. Irradiation influenced only the peak of ~1454 cm-1. It is concluded that phototherapies influences bone repair in cases of thyroid diseases.
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Amanda P. Soares, Tania T. Rodriguez, Luiz G. P. Soares, Jean Nunes dos Santos, Landulfo Silveira Jr., and Antonio Luiz B. Pinheiro "The effect of phototherapies on bone repair of euthyroid and hypothyroid rats: Raman spectroscopic study", Proc. SPIE 10582, Laser Florence 2017: Advances in Laser Medicine, 1058207 (12 April 2018); https://doi.org/10.1117/12.2315639
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