7 December 2017 AOTF-based optical system of a microscope module for multispectral imaging techniques
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Proceedings Volume 10592, Biophotonics—Riga 2017; 105920H (2017) https://doi.org/10.1117/12.2297614
Event: The Second International Conference "Biophotonics-Riga 2017", 2017, Riga, Latvia
Abstract
Multi-spectral imaging techniques are widely used in microscopy for many applications. One of the most widespread spectral elements for this purpose is an imaging acousto-optic tunable filter (AOTF). AOTF-based contrast visualization is especially effective when used together with other imaging techniques. Simultaneous utilization of two or more imagers requires optical coupling to provide point-to-point matching of the obtained images. Small linear and angular aperture of AOTF additionally hampers the development of multi-sensor imaging systems for microscopy. In this paper, we present a compact optical system which allows to integrate AOTF-based spectral imager into the schemes of conventional microscopes and provide high-quality spectral image of the same scale as in another, for example, wideband channel. The effectiveness of the proposed approach is demonstrated experimentally.
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Olga Polschikova, Alexander Machikhin, Vladislav Batshev, Alina Ramazanova, Artyom Belov, Vitold Pozhar, "AOTF-based optical system of a microscope module for multispectral imaging techniques", Proc. SPIE 10592, Biophotonics—Riga 2017, 105920H (7 December 2017); doi: 10.1117/12.2297614; https://doi.org/10.1117/12.2297614
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