7 December 2017 AOTF-based optical system of a microscope module for multispectral imaging techniques
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Proceedings Volume 10592, Biophotonics—Riga 2017; 105920H (2017) https://doi.org/10.1117/12.2297614
Event: The Second International Conference "Biophotonics-Riga 2017", 2017, Riga, Latvia
Abstract
Multi-spectral imaging techniques are widely used in microscopy for many applications. One of the most widespread spectral elements for this purpose is an imaging acousto-optic tunable filter (AOTF). AOTF-based contrast visualization is especially effective when used together with other imaging techniques. Simultaneous utilization of two or more imagers requires optical coupling to provide point-to-point matching of the obtained images. Small linear and angular aperture of AOTF additionally hampers the development of multi-sensor imaging systems for microscopy. In this paper, we present a compact optical system which allows to integrate AOTF-based spectral imager into the schemes of conventional microscopes and provide high-quality spectral image of the same scale as in another, for example, wideband channel. The effectiveness of the proposed approach is demonstrated experimentally.
© (2017) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Olga Polschikova, Olga Polschikova, Alexander Machikhin, Alexander Machikhin, Vladislav Batshev, Vladislav Batshev, Alina Ramazanova, Alina Ramazanova, Artyom Belov, Artyom Belov, Vitold Pozhar, Vitold Pozhar, } "AOTF-based optical system of a microscope module for multispectral imaging techniques", Proc. SPIE 10592, Biophotonics—Riga 2017, 105920H (7 December 2017); doi: 10.1117/12.2297614; https://doi.org/10.1117/12.2297614
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