18 January 2018 Light scattering method to measure red blood cell aggregation during incubation
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Proceedings Volume 10612, Thirteenth International Conference on Correlation Optics; 106121C (2018) https://doi.org/10.1117/12.2305388
Event: Thirteenth International Conference on Correlation Optics, 2017, Chernivtsi, Ukraine
Abstract
Red blood cell (RBC) aggregation can be observed both in vivo as well as in vitro. This process is a cause of alterations of blood flow in microvascular network. Enhanced RBC aggregation makes oxygen and nutrients delivery difficult. Measurements of RBC aggregation usually give a description of the process for a sample where the state of a solution and cells is well-defined and the system reached an equilibrium. Incubation of RBCs in various solutions is frequently used to study the effects of the solutions on the RBC aggregation. The aggregation parameters are compared before and after incubation while the detailed changes of the parameters during incubation remain unknown. In this paper we have proposed a method to measure red blood cell aggregation during incubation based on the well-known technique where backscattered light is used to assess the parameters of the RBC aggregation. Couette system consisting of two cylinders is adopted in the method. The incubation is observed in the Couette system. In the proposed method following sequence of rotations is adapted. Two minutes rotation is followed by two minutes stop. In this way we have obtained a time series of back scattered intensity consisting of signals respective for disaggregation and aggregation. It is shown that the temporal changes of the intensity manifest changes of RBC aggregation during incubation. To show the ability of the method to assess the effect of incubation time on RBC aggregation the results are shown for solutions that cause an increase of RBC aggregation as well as for the case where the aggregation is decreased.
© (2018) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
B. Grzegorzewski, B. Grzegorzewski, A. Szołna-Chodór, A. Szołna-Chodór, J. Baryła, J. Baryła, D. Drężek, D. Drężek, } "Light scattering method to measure red blood cell aggregation during incubation", Proc. SPIE 10612, Thirteenth International Conference on Correlation Optics, 106121C (18 January 2018); doi: 10.1117/12.2305388; https://doi.org/10.1117/12.2305388
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