12 January 2018 Characterizing the hydration state of L-threonine in solution using terahertz time-domain attenuated total reflection spectroscopy
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Proceedings Volume 10623, 2017 International Conference on Optical Instruments and Technology: IRMMW-THz Technologies and Applications; 106230N (2018) https://doi.org/10.1117/12.2295512
Event: International Conference on Optical Instruments and Technology 2017, 2017, Beijing, China
Abstract
The hydration of biomolecules is closely related to the dynamic process of their functional expression, therefore, characterizing hydration phenomena is a subject of keen interest. However, direct measurements on the global hydration state of biomolecules couldn’t have been acquired using traditional techniques such as thermodynamics, ultrasound, microwave spectroscopy or viscosity, etc. In order to realize global hydration characterization of amino acid such as L-threonine, terahertz time-domain attenuated total reflectance spectroscopy (THz-TDS-ATR) was adopted in this paper. By measuring the complex permittivity of L-threonine solutions with various concentrations in the THz region, the hydration state and its concentration dependence were obtained, indicating that the number of hydrous water decreased with the increase of concentration. The hydration number was evaluated to be 17.8 when the molar concentration of L-threonine was 0.34 mol/L, and dropped to 13.2 when the molar concentration increased to 0.84 mol/L, when global hydration was taken into account. According to the proposed direct measurements, it is believed that the THz-TDS-ATR technique is a powerful tool for studying the picosecond molecular dynamics of amino acid solutions.
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Huachuan Huang, Qiao Liu, Liguo Zhu, Zeren Li, "Characterizing the hydration state of L-threonine in solution using terahertz time-domain attenuated total reflection spectroscopy", Proc. SPIE 10623, 2017 International Conference on Optical Instruments and Technology: IRMMW-THz Technologies and Applications, 106230N (12 January 2018); doi: 10.1117/12.2295512; https://doi.org/10.1117/12.2295512
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