High Resolution Flow Cytometry As An Analytical Tool For Characterizing Aquatic Bacteria

Don K. Button; Betsy R. Robertson

doi:10.1117/12.951910

13 June 1989 High Resolution Flow Cytometry As An Analytical Tool For Characterizing Aquatic Bacteria

Don K. Button, Betsy R. Robertson

Author Affiliations +

Proceedings Volume 1063, New Technologies in Cytometry; (1989) https://doi.org/10.1117/12.951910
Event: OE/LASE '89, 1989, Los Angeles, CA, United States

Abstract

Flow cytometry offers an ideal new tool for evaluating aquatic bacteria because of the many analyses that can be made on each cell, sensitivity exceeding that of the light microscope, speed, simplicity of sample preparation, ability to sort, and the ideal density of bacterial populations in natural systems for direct analysis. Capabilities are demonstrated here by resolving the comparatively small bacteria in seawater samples as well as filter-fractionated subpopulations of them. Histograms from under-ice lakewater indicated even smaller and more difficult to resolve organisms; these are shown as well. Several suggestions are made for improving the effectiveness of flow cytometry for bacterial analyses. Resulting enhanced sensitivity should increase the precision with which flow cytometric measurements of aquatic bacteria can be made and improve the already substantial utility of this instrumentation as a diagnostic tool for the larger and better-known terrestrial symbiotic and pathogenic types.

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Don K. Button and Betsy R. Robertson "High Resolution Flow Cytometry As An Analytical Tool For Characterizing Aquatic Bacteria", Proc. SPIE 1063, New Technologies in Cytometry, (13 June 1989); https://doi.org/10.1117/12.951910

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