15 May 2018 Capture of bacterial pathogens in liquid streams by multiple layers of phage based bio-molecular filter
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Abstract
Foodborne illness is a common public health problem because food can be contaminated with pathogens at any point in the farm-to-table continuum. This paper presents a method of capturing a quantity of a specific bacterial pathogen in a large volume of liquid using a biomolecular recognition filter. The filter consists of support frames made of a soft magnetic material and solenoid coils for magnetization/demagnetization of the frames. This filter is a planar, multi-layered arrangement of strip-shaped, phage-immobilized magnetoelastic (ME) biosensors that are magnetically held and arrayed on the filter frames. As a large volume of liquid passes through the biomolecular filter, the pathogen of interest is captured by the phage immobilized ME biosensors. This biomolecular filter is designed to capture a specific pathogen and allow non-specific debris to pass, thus avoiding a common clogging issue in conventional bead filters. In this work, single layer, double layers and triple layers of filter were test to capture Salmonella Typhimurium in a large volume of water. The effects of multiplication of filter layers on Salmonella capture efficiency will be discussed.
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Songtao Du, Yuzhe Liu, Jianguo Xi, I-Hsuan Chen, Xu Lu, Shin Horikawa, Tung-shi Huang, Bryan A. Chin, "Capture of bacterial pathogens in liquid streams by multiple layers of phage based bio-molecular filter", Proc. SPIE 10665, Sensing for Agriculture and Food Quality and Safety X, 106650O (15 May 2018); doi: 10.1117/12.2304535; https://doi.org/10.1117/12.2304535
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