22 May 2018 The technology of laser fabrication of cell 3D scaffolds based on proteins and carbon nanoparticles
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Abstract
The technology of cell 3D scaffolds laser fabrication is developed. 3D scaffolds are designed to repair osteochondral defects, which are poorly restored during the organism’s life. The technology involves the use of an installation, the laser beam of which moves along a liquid nanomaterial and evaporates it layer by layer. Liquid nanomaterial consists of the water-protein (collagen, albumin) suspension with carbon nanoparticles (single-walled carbon nanotubes). During laser irradiation, the temperature in the region of nanotubes defects increases and nanotubes are combined into the scaffold. The main component of installation is a continuous laser operating at wavelengh of 810 nm. The laser beam moves along 3 coordinates, which makes it possible to obtain samples of the required geometric shape. The internal and surface structure of the samples at the micro- and nanoscale levels were studied using the X-ray microtomography and scanning electron microscopy. In vitro studies of cell growth during 48 and 72 hours demonstrated the ability of cell 3D scaffolds to support the proliferation of osteoblasts and chondroblasts. Using fluorescence and atomic force microscopy, it was found that the growth and development of cells on a sample with a larger concentration of nanotubes occurred faster compared to samples with a smaller concentration of nanotubes.
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Alexander Gerasimenko, Alexander Gerasimenko, Natalia Zhurbina, Natalia Zhurbina, Ulyana Kurilova, Ulyana Kurilova, Aleksandr Polokhin, Aleksandr Polokhin, Dmitry Ryabkin, Dmitry Ryabkin, Mikhail Savelyev, Mikhail Savelyev, Irina Suetina, Irina Suetina, Marina Mezentseva, Marina Mezentseva, Levan Ichkitidze, Levan Ichkitidze, Dmitry Ignatov, Dmitry Ignatov, Mario Alberto Garcia-Ramirez, Mario Alberto Garcia-Ramirez, Jose Valentin Guzman Gonzalez, Jose Valentin Guzman Gonzalez, Vitaliy Podgaetsky, Vitaliy Podgaetsky, "The technology of laser fabrication of cell 3D scaffolds based on proteins and carbon nanoparticles", Proc. SPIE 10675, 3D Printed Optics and Additive Photonic Manufacturing, 1067510 (22 May 2018); doi: 10.1117/12.2306792; https://doi.org/10.1117/12.2306792
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