Quantitative phase imaging of low-cost digital in-line holography in comparison with off-axis digital holographic microscopy

Hanu Phani Ram G.; Renu John

doi:10.1117/12.2310077

24 May 2018 Quantitative phase imaging of low-cost digital in-line holography in comparison with off-axis digital holographic microscopy

Hanu Phani Ram G., Renu John

Author Affiliations +

Proceedings Volume 10677, Unconventional Optical Imaging; 106773C (2018) https://doi.org/10.1117/12.2310077
Event: SPIE Photonics Europe, 2018, Strasbourg, France

Abstract

Digital holographic microscopy (DHM) is a quantitative phase imaging (QPI) modality, which retrieves 3D object phase information. The quantification of subcellular features within the object is possible. Its single-shot hologram recording feature makes it suitable for real-time imaging applications. This paper discusses QPI capability of LED-based digital inline holographic microscopy (LDHM), which has gained much attention for its portability, cost-effective features. However, the twin image artifact is present in the inline setup. Though several twin image reduction and elimination methods are developed, the exact phase quantification is always a challenge. Original phase information may be lost after elimination of twin image. There is always a trade-off between twin-image elimination and QPI of inline microscopy setup. This paper discusses the QPI capability of LDHM in comparison with the conventional off-axis DHM. Further, the results of phase objects using both the methods are studied.

Citation Download Citation

Hanu Phani Ram G. and Renu John "Quantitative phase imaging of low-cost digital in-line holography in comparison with off-axis digital holographic microscopy", Proc. SPIE 10677, Unconventional Optical Imaging, 106773C (24 May 2018); https://doi.org/10.1117/12.2310077

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KEYWORDS

Holography

Digital holography

Holograms

3D image reconstruction

Microscopy

Phase imaging

Reconstruction algorithms

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