24 May 2018 Quantitative phase imaging of low-cost digital in-line holography in comparison with off-axis digital holographic microscopy
Author Affiliations +
Abstract
Digital holographic microscopy (DHM) is a quantitative phase imaging (QPI) modality, which retrieves 3D object phase information. The quantification of subcellular features within the object is possible. Its single-shot hologram recording feature makes it suitable for real-time imaging applications. This paper discusses QPI capability of LED-based digital inline holographic microscopy (LDHM), which has gained much attention for its portability, cost-effective features. However, the twin image artifact is present in the inline setup. Though several twin image reduction and elimination methods are developed, the exact phase quantification is always a challenge. Original phase information may be lost after elimination of twin image. There is always a trade-off between twin-image elimination and QPI of inline microscopy setup. This paper discusses the QPI capability of LDHM in comparison with the conventional off-axis DHM. Further, the results of phase objects using both the methods are studied.
© (2018) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Hanu Phani Ram G., Hanu Phani Ram G., Renu John, Renu John, } "Quantitative phase imaging of low-cost digital in-line holography in comparison with off-axis digital holographic microscopy", Proc. SPIE 10677, Unconventional Optical Imaging, 106773C (24 May 2018); doi: 10.1117/12.2310077; https://doi.org/10.1117/12.2310077
PROCEEDINGS
6 PAGES


SHARE
Back to Top