24 May 2018 Axial scanning and spherical aberration correction in confocal microscopy employing an adaptive lens
Author Affiliations +
Abstract
We present a fluid-membrane lens with two piezoelectric actuators that offer versatile, circular symmetric lens surface shaping. A wavefront-measurement-based control system ensures robustness against creeping and hysteresis effects of the piezoelectric actuators. We apply the adaptive lens to correct synthetic aberrations induced by a deformable mirror. The results suggest that the lens is able to correct spherical aberrations with standard Zernike coefficients between 0 μm and 1 μm, while operating at refractive powers up to about 4m-1. We apply the adaptive lens in a custom-built confocal microscope to allow simultaneous axial scanning and spherical aberration tuning. The confocal microscope is extended by an additional phase measurement system to include the control algorithm. To verify our approach, we use the maximum intensity and the axial FWHM of the overall confocal point spread function as figures of merit. We further discuss the ability of the adaptive lens to correct specimen-induced aberrations in a confocal microscope.
Conference Presentation
© (2018) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Katrin Philipp, Katrin Philipp, Florian Lemke, Florian Lemke, Matthias C. Wapler, Matthias C. Wapler, Nektarios Koukourakis, Nektarios Koukourakis, Ulrike Wallrabe, Ulrike Wallrabe, Jürgen W. Czarske, Jürgen W. Czarske, } "Axial scanning and spherical aberration correction in confocal microscopy employing an adaptive lens ", Proc. SPIE 10679, Optics, Photonics, and Digital Technologies for Imaging Applications V, 106790E (24 May 2018); doi: 10.1117/12.2307377; https://doi.org/10.1117/12.2307377
PROCEEDINGS
6 PAGES + PRESENTATION

SHARE
Back to Top