9 May 2018 Fast fluorescence lifetime determination with an ASIC detector unit
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Abstract
Fluorescence lifetime determination is widely utilized for bioscience research and analysis. The fluorescence stimulation in conventional systems is usually done with expensive picosecond laser systems. We present a cost-effective 370 nm LED based excitation module and a detection unit based on a Silicon Photomultiplier (SiPM). The functionality of the excitation module as well as the detection module is demonstrated with the fluorescence dye ATTO 390.

For a fast analysis of the fluorescence signal detected by the SiPM, we developed an ASIC for fluorescence histogram recording. The ASIC determines the time between excitation pulse and incoming fluorescence photon with an accuracy of about 80 ps. The ASIC blind time after the excitation pulse is configurable. The determined time is saved in bins. The width of the bins is programmable. Output of the ASIC is a histogram with the counted amount of photons at the different times after excitation. This histogram equals the fluorescence response of the dye. The fluorescence lifetime can be calculated out of this histogram.
Conference Presentation
© (2018) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Ch. Möller, V. Körner, R. Matthes, Ch. Heinze, H.-G. Ortlepp, W. Altermann, T. Schildbach, M. Winkler, D. Buchweitz, M. Götz, T. Ortlepp, "Fast fluorescence lifetime determination with an ASIC detector unit", Proc. SPIE 10680, Optical Sensing and Detection V, 1068009 (9 May 2018); doi: 10.1117/12.2306752; https://doi.org/10.1117/12.2306752
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