14 May 2018 Physical parameters of ultrawide band laser sources and their impacts on multiphoton imaging
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Abstract
The ability of a low repetition rate pulsed-nanosecond supercontinuum source (SC) have been recently demonstrated as able to generate multiphoton images with a quality similar as those obtained with a standard titanium sapphire source. In this publication, the phenomenon involved is theoretically and experimentally studied and detailed. First, the interest of a SC source for multiphoton microscopy (MPM) is reminded and this point is illustrated with a numerical comparison of the effect of the combination of pulse duration and repetition rate of the source on the TPA of photons by a fluorophore. Then, a theoretical comparison of excitation sources used in MPM is presented. Finally, an experimental focus on the effect of the repetition rate on the image quality and the axial and lateral resolutions are highlighted. The results pave the way for simultaneous multiplex MPM.
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Laetitia Magnol, Laetitia Magnol, Véronique Blanquet, Véronique Blanquet, Claire Lefort, Claire Lefort, "Physical parameters of ultrawide band laser sources and their impacts on multiphoton imaging", Proc. SPIE 10684, Nonlinear Optics and its Applications 2018, 106841B (14 May 2018); doi: 10.1117/12.2303719; https://doi.org/10.1117/12.2303719
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