Implementation of stimulated Raman losses and stimulated Raman gain microscopy using three femtosecond laser sources

Rajeev Ranjan; Annalisa D'Arco; Maria Antonietta Ferrara; Maurizio Indolfi; Michele Larobina; Luigi Sirleto

doi:10.1117/12.2315967

14 May 2018 Implementation of stimulated Raman losses and stimulated Raman gain microscopy using three femtosecond laser sources

Rajeev Ranjan, Annalisa D'Arco, Maria Antonietta Ferrara, Maurizio Indolfi, Michele Larobina, Luigi Sirleto

Author Affiliations +

Proceedings Volume 10684, Nonlinear Optics and its Applications 2018; 106842J (2018) https://doi.org/10.1117/12.2315967
Event: SPIE Photonics Europe, 2018, Strasbourg, France

Abstract

Stimulated Raman scattering microscopy allows vibrational contrast mechanism for imaging with high spectral and spatial resolution along with three-dimensional sectioning. In this paper, the implementation of a Stimulated Raman Scattering microscope (SRSM), obtained by the integration of a femtosecond SRS spectroscopy set-up with an optical microscope equipped with a scanning unit, is described. Femtosecond Stimulated Raman Scattering microscope is equipped with three femtosecond laser sources: a Ti:Sapphire (Ti:Sa), a synchronized optical parametric oscillator (SOPO) and a frequency converters for ultrafast lasers, i.e. a second harmonic generator optimized for the SOPO. The proposed implementation allows to cover all the regions of Raman spectra, taking advantage of two different laser combinations. The first, Ti:Sa and SHG laser combination can cover in SRL modality the fingerprint region (500 − 1700 cm⁻¹ ) and the silent region. The second, Ti:Sa and OPO, can cover the C-H region or O−H region (2800 − 3200 cm⁻¹ ) in SRG modality. In order to demonstrate its successful realization Stimulated Raman Gain (SRG) and Stimulated Raman Losses (SRL) images of polystyrene beads are reported and discussed.

Citation Download Citation

Rajeev Ranjan, Annalisa D'Arco, Maria Antonietta Ferrara, Maurizio Indolfi, Michele Larobina, and Luigi Sirleto "Implementation of stimulated Raman losses and stimulated Raman gain microscopy using three femtosecond laser sources", Proc. SPIE 10684, Nonlinear Optics and its Applications 2018, 106842J (14 May 2018); https://doi.org/10.1117/12.2315967

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

;

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
8 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Raman spectroscopy

Microscopes

Microscopy

Optical parametric oscillators

Femtosecond phenomena

Second-harmonic generation

Spectroscopy

Show All Keywords

Keywords/Phrases

Search In:

Publication Years